Biomedical Engineering Seminar - 4 May 2023

You are welcome to attend in physical or digital format. A lunch sandwich will be served for those attending in person. 

Invited Speaker: Dr. Niklas Sandström, Dept. of Applied Physics, KTH Royal Institute of Technology,  SciLifeLab

Title:  Longitudinal screening and high-resolution imaging in microwells of 2D/3D live immune cell assays 

When: 4th of May,  12:15-13:15 (CET)

Where: In person  (Å2001 <https://link.mazemap.com/HLKgLJb2> )  or on Zoom (https://uu-se.zoom.us/j/62953800151)

Please register no later than 13:00 on 3rd of May if you would like to receive a sandwich:  https://doit.medfarm.uu.se/bin/kurt3/kurt/91376

Abstract: Single-cell technologies have revolutionized research areas such as cancer and immune cell biology, enabling extensive characterization of phenotypic profiles of single cells revealing heterogeneity in supposed homogenous cell populations. Although such techniques, like transcriptomics and flow cytometry, are powerful tools for single-cell analysis, they are not capable of examining functional differences and responses to any great extent. In contrast, imaging allows longitudinal live cell assays with single-cell resolution. The use of multiwell plates enables high-content screening in a multitude of liquid conditions, but detailed functional studies of single-cells over time remains challenging. To address this issue, we have developed a range of microwell chips for live cell imaging assays with high optical quality where cells are confined for longitudinal studies. Our microwell platform enables screening and high-resolution imaging, which we utilize to investigate individual functional responses of natural killer cells in contact with tumor cells in different conditions. Our live cell assays in microwells involves 2D cell cultures as well as 3D tumor spheroid cultures and are aimed at applications within cell therapy and precision medicine. 

Biography:

Niklas is a researcher at the Div. of Biophysics, Dept. of Applied Physics, KTH Royal Institute of Technology, SciLifeLab, in Stockholm, Sweden. He has a M.Sc. in Engineering Biology and a Ph.D. in Micro and Nanosystems/Electrical Measurements. Currently, he is leading the development of a microwell chip platform for efficient screening and high-resolution imaging of live cell assays and related research projects. With a keen interest in imaging, he also teaches photography for Media Technology students at KTH and works as an operations officer for the National Microscopy Infrastructure (NMI) in Sweden.